Advances in gene expression control via active translational coupling
BIOFAB has developed a method that allows for reliable functional coupling of prokaryotic transcription and translation control elements with user-specific coding sequences for genes of interest. The BIOFAB team engineered a collection of standard biological parts that implements the method across a range of transcription and translation levels, and with native and also a phage RNA polymerase. For the genes tested in the system so far, BIOFAB achieved >90% predictability of gene expression to within a two-fold protein concentration of the target window. Team Leader Vivek Mutalik announced the work during Cambridge Healthtech Institute's Third Annual Engineering Genes, Vectors, Constructs and Clones Conference on January 9, 2012.